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Fungal Blood Culture (8000100359)
Test Mnemonic:

F MBLD For fungus only

C MAF for AFB and fungus combined

Specimen Requirements:
Collection:

https://www.medialab.com/dv/dl.aspx?d=1412131&dh=21147&u=87474&uh=bc5aa

Do not touch phlebotomy site after cleansing to prevent false positive culture growth. Use a syringe or butterfly to collect 1-5 mL of blood. Inject blood into the alchohol-cleaned MycoF/Lytic bottle. 

Container:

Myco/F Lytic  bottle (red cap) 

Minimum Volume:

3 mL is the minimum volume for optimal recovery.

Storage/Transport:

Room temperature; Transport to Microbiology Lab ASAP

Specimen Preparation:

The specimen must be collected using sterile technique to reduce the chance of contamination. The range of blood volume for optimum recovery is 3-5 mL. Prior to inoculation, bottles should be examined for evidence of contamination such as cloudiness, bulging or depressed septum, or leakage. 

Stability:

Transport to Microbiology Lab ASAP

Causes for Rejection:

Mislabeled, Under-labeled, or improper transport/ collection.

Specimens must be labeled. Loose labels placed in the bag or outside of the bag will not be accepted and will be considered under-labeled. 

Reference Range:

No Fungi Isolated. 

Turnaround Time:

6 weeks

Methodology:

Bottles are continuously agitated and incubated in the BD BACTEC FX. Decreases in oxygen due to microorganism metabolism and growth will trigger positive bottle alert. 

Performed:

Clinical Microbiology

Synonyms:

Blood culture;fungus culture; fungal blood culture;fungal septicemia; septicemia

Clinical Indication:

Suspected fungal blood stream infection. 

Patient Preparation :

Prep patient and follow blood culture guidelines according to UTMB's evidence-based practices and policies. 

CPT 4 Code:

<p style="line-height: normal; margin-bottom: 0pt"> <font face="Georgia">87103, additional codes if indicated</font></p>

Note:

<p> <font face="Georgia" size="2">Positive culture results are considered a critical value and notification will be performed upon recognition of the positive result. </font></p> <p> <font face="Georgia" size="2">Order C MAF if requesting cultures for both mycobacteria and fungus and F MBLD is only for fungus.</font></p> <p> <font face="Georgia" size="2"><font face="Georgia" size="2">Collect specimens using aseptic technique since contamination may lead to false positive results. Due to recovery time, it is not recommended to collect more than two blood culture fungal vials within five days. The Aerobic blood culture vials recover yeast.</font></font></p> <p style="line-height: normal; margin-bottom: 0pt"> <font face="Georgia" size="2"><small><font face="Georgia" size="2"><strong>DO NOT COVER THE BOTTLE BAR CODE LABEL</strong></font></small></font></p>

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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