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Von Willebrand Disease (vWD) Evaluation (LAB002011)
Test Mnemonic:

vWD Eval
Specimen Requirements:

Fill within ±10% from stated capacity. Immediately after draw, gently invert 3-4 times.
Test Included:

vWF antigen, vWF activity, Fibrinogen, Factor VIII activity, PLT count, Dagnostic Management team eval,

If indicated vWF Ristoctin Co-Factor, vWF Multimers, 
Collection:

Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume. 
Container:

Both

Blue-topped (3.2% sodium-citrate) tube

EDTA (Lavender-top tube)
Minimum Volume:

3 mL plasma
Storage/Transport:

Blue Top:

If delivery time is to be greater than 4 hours from time of draw, centrifuge the specimen for 15 minutes at 2000-2500g

  • Transfer plasma to 12x75 plastic tube
  • Cap tube and re-spin  for 15 minutes at 2000-2500g
  • CAREFULLY remove from centrifuge without disturbing any platelets and/or cell pellets that might be on the bottom or sides of the tube.
  • Carefully transfer plasma into freezer tubes (plastic screw-top cryo-tubes) for testing or freezing.

Lavender Top:

Room Temperature 4 hours .  If testing cannot be completed  with in 4 hours sample must be refrigerated.
Stability:

Blue Top:

Whole blood 4 hours at room temperature. Plasma 8 hours at 20+/- 5°C.  Frozen at -20 °C 2 weeks.

Lavender Top:

Stable Refrigerated for 36 hours.
Causes for Rejection:

QNS, clotted, severely hemolyzed specimen, wrong tube, high hematocrit (> 55%), sample identification error, sample processed/transported, and/or stored improperly.
Reference Range:

See each individual test for Ranges
Turnaround Time:

 

The hours of operation are Monday – Friday from 8:00 am to 4:00 pm with an expected TAT of 4 hours. STAT requests after business hours will be evaluated and handled by the on-call staff member.

 
Methodology:

See each individual tests for Methodology
Performed:
Lab:

CLS Specialty Lab

Click to view CLS Specialty Lab website 

PLT count- UTMB Hematology 

Ristocetin Co-Factor and vWF Multimers- send out 
When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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