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Antiphospholipid Antibody Evaluation ([LAB002028])
Test Mnemonic:

ANTIPHOSPHOLIPID ANTIBODY TESTS AND EVALUATION

 
Specimen Requirements:
Test Included:

Antiphospholipid Antibody Evaluation - Lt Blue 

PT

APTT

Lupus Anticoagulation Evaluation

 

Antiphospholipid Antibody Evaluation - SST (send out)

Anticardiolipin Antibodies

Anti B2GPI antibodies
Collection:

Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume. 
Container:

Blue-topped (3.2% sodium-citrate) tube

SST tube
Minimum Volume:

3 mL of Plasma

1 mL of Serum
Storage/Transport:

Blue-topped (3.2% sodium-citrate) tube 

Shipping/Handling instructions:                                                                           

  1. If delivery time is to be greater than 2 hours from time of draw, centrifuge the specimen for 15 minutes at 2000-2500g.
  2. Transfer plasma to 12x75 plastic tube.
  3. Cap tube and re-spin for 15 minutes at 2000-2500g.
  4. Remove from centrifuge without disturbing any platelet and/or cell pellets that might be on the bottom or sides of the tube.
  5. Carefully transfer plasma into freezer tubes (plastic screw-top cryo-tubes) for testing or freezing and be sure to tape the patient's label onto the tube.
  6. Store transport tube in freezer (-20, or -70C) in an upright position.
SST tube
Separate serum from cells within 2 h of collection; avoid repeated freeze/thaw cycles.
Stability:

Blue-topped (3.2% sodium-citrate) tube  

Ambient - 2 hours; Refrigerated - Unacceptable; Frozen -20oC – 2 weeks, at -70oC - 6 months 

 

If the testing is not completed within 2 hours, platelet-poor plasma should be removed without disturbing the sedimented cells (buff-coat) and frozen at -20oC or below for short-term storage (up to 2 weeks), or -70oC for 6 months.

 
Causes for Rejection:
  • Clotted and hemolyzed plasma samples are unacceptable and must be redrawn.
  • Wrong tube or anticoagulant.
  • Wrong anticoagulant ratio (over or underfilled tubes).
  • Not centrifuged within the acceptable time after collection.
  • Whole blood or plasma refrigerated or placed on ice prior to testing.
  • Unlabeled.
  • Mislabeled.
 

 
Reference Range:

Refer to specific test reference range
Turnaround Time:

Monday through Friday
Methodology:

Refer to specific test methodology
Performed:

Antiphospholipid Antibody Evaluation - Lt Blue: Hematopathology

Antiphospholipid Antibody Evaluation - SST: Send out
Synonyms:

ANTIPHOSPHOLIPID ANTIBODY TESTS AND EVALUATION

ANTIPHOSPHOLIPID ANTIBODY EVALUATION PANEL

 
CPT 4 Code:

Refer to specific test CPT code
When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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