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avitiNex-Gen Core: Next Generation Sequencing Core Facility

UTMB’s Next Generation Sequencing (NGS) Core provides high throughput sequencing to UTMB investigators and others using dedicated Element Biosciences’ Aviti sequencing instrument and Illumina NextSeq 2000, NextSeq 550 (this will be retired by the end of FY 24) and MiniSeq instrument.  Aviti offers lower cost and higher Qscore, while Illumina platforms are still the mainsteam technologies on the market.  The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq), and DNA (genomic and amplicon-derived). Library quality and quantity is assessed using Agilent Bioanalyzer and qPCR. Multiple platforms offer choices of different technologies and cost structures.  Libraries prepared for Illumina sequencing can be sequenced on both technological platforms.  In addition to short read sequencing, the core can also offer long read sequencing support.  Please contact core if you are interested in long read sequencing (RNA isoform sequencing, full length 16s rRNA sequencing, and de novo genome assembly). 

What can you do with Next Generation Sequencing?
  • ChIP-Seq
  • De novo assembly
  • Variant identification
  • SNPs
  • Transcriptome (reference-guided or de novo)
  • miRNA and small ncRNA sequencing
  • Quantitative - RNA-Seq expression analysis
  • Exome Sequencing (Cancer or Custom Panels)
Request Forms

ISC Instructions

Next Generation Sequencing Costs: October 1,2024
PlatformRun TypeLane/Flowcell*Estimated ReadsCost
Aviti    
 PE300Lane50M$1,475
 PE300Flowcell100M$2,350
 PE300Lane150M$1,850
 PE300Flowcell300M$3,100
 PE150Lane125M$775
 PE150Flowcell250M$1,250
 PE75Lane250M$875
 PE75Flowcell500M$1,450
 PE75Lane500M$1,200
 PE75Flowcell1B$2,100
 PE150Lane250M$1,050
 PE150Flowcell500M$1,800
 PE150Lane500M$1,400
 PE150Flowcell1B$2,500

* Aviti flowcells have two lanes that can be used for different libraries in the same run.  For per lane sequencing, there will be a slight wait time to have two libraries for the same run type (same read length, same index read length, unless you have two pools).

NextSeq 2000  
SE 100, XLEAP100M$1,225
PE 150, XLEAP100M$1,420
PE 300, XLEAP100M$2,025
SE 100, XLEAP400M$1,556
PE 100, XLEAP400M$2,150
PE 150, XLEAP400M$2,250
PE 300, XLEAP300M$3,520
SE 50 1200M$2,700
SE 100, XLEAP1200M$2,200
PE 100, XLEAP1200M$3,220
PE 150, XLEAP1200M$3,300
SE 50, XLEAP1800M$2,450
SE 100, XLEAP1800M$2,900
PE 100, XLEAP1800M$4,000
PE 150, XLEAP1800M$4,300

M, million; SE, single end; PE, paired end

NGS Data Analysis                       $85/hr

Library Cost 
RNA-Seq Non-Stranded, no dT selection$276.00
RNA-Seq Non-Stranded & dT Selected$282.00
RNA-Seq Stranded & dT Selected$304.00
RNA-Seq RiboZero rRNA Depletion$373.00
ChIP-Seq$269.00
DNA-Seq (Covaris)$274.00
DNA-Seq  Nextera Flex$278.00
microRNA (Small RNA)$296.00

Staff:

Jill K. Thompson
Haiping Hao, PhD

Location: 2.210 Basic Science Bldg.
Contact: hahao@utmb.edu
Phone: (409) 772-6349

Please contact Dr. Hao to discuss your sequencing project.